2 well chamber slides Search Results


8 well chamber slides  (Eppendorf AG)
99
Eppendorf AG 8 well chamber slides
8 Well Chamber Slides, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96 well ecis plates  (Applied BioPhysics)
94
Applied BioPhysics 96 well ecis plates
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
96 Well Ecis Plates, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eight-well labtek chamber slide  (Labtek)
90
Labtek eight-well labtek chamber slide
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
Eight Well Labtek Chamber Slide, supplied by Labtek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2-well labtek minimal levels of the wild-type er mrna transcript were chamber slides  (Labtek)
90
Labtek 2-well labtek minimal levels of the wild-type er mrna transcript were chamber slides
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
2 Well Labtek Minimal Levels Of The Wild Type Er Mrna Transcript Were Chamber Slides, supplied by Labtek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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8-well chamber lides  (Fisher Scientific)
90
Fisher Scientific 8-well chamber lides
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
8 Well Chamber Lides, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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8-well falcon cultureslides  (Corning Life Sciences)
90
Corning Life Sciences 8-well falcon cultureslides
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
8 Well Falcon Cultureslides, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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8-well culture slides  (SPL Life Sciences)
90
SPL Life Sciences 8-well culture slides
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
8 Well Culture Slides, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2-well chamber slides fisher scientific 12-565-16  (Fisher Scientific)
90
Fisher Scientific 2-well chamber slides fisher scientific 12-565-16
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
2 Well Chamber Slides Fisher Scientific 12 565 16, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4-well chamber slides  (SPL Life Sciences)
90
SPL Life Sciences 4-well chamber slides
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
4 Well Chamber Slides, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4-well chamber slides 354577  (Corning Life Sciences)
90
Corning Life Sciences 4-well chamber slides 354577
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
4 Well Chamber Slides 354577, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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8-chamber cell culture slide cat. no. 229168  (Ultident Scientific)
90
Ultident Scientific 8-chamber cell culture slide cat. no. 229168
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
8 Chamber Cell Culture Slide Cat. No. 229168, supplied by Ultident Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagen-coated cell-culture chamber slide  (Becton Dickinson)
90
Becton Dickinson collagen-coated cell-culture chamber slide
Schematic explaining <t>ECIS</t> theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.
Collagen Coated Cell Culture Chamber Slide, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Schematic explaining ECIS theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.

Journal: International Journal of Molecular Sciences

Article Title: Melanoma Cells Produce Large Vesicular-Bodies That Cause Rapid Disruption of Brain Endothelial Barrier-Integrity and Disassembly of Junctional Proteins

doi: 10.3390/ijms24076082

Figure Lengend Snippet: Schematic explaining ECIS theory. ( A ) Endothelial cells grown on the gold-plated electrodes of the ECIS plate that are coated with collagen, forming a confluent monolayer. The monolayer is formed via intercellular junctions and junctions with the collagen substrate, creating the paracellular and basolateral junctions, respectively. The formation of these junctions prevents high current flow from the electrode through the cells, where the red and green arrows represent the paracellular (Rb) and basolateral (alpha) current flow, respectively. This maintained electrical resistance is shown in graph C (Black). ( B ) The endothelial monolayer following treatment with factors that disrupt the endothelial barrier, such as melanoma cells or melanoma vesicular-bodies; the junctions weaken, allowing for greater current flow through the cells. This results in a decreased electrical resistance, shown in graph ( C ) (Blue). ( C ) A model ECIS graph depicting the plateau in resistance formed prior to treatment, indicating a fully formed endothelial barrier, and the corresponding results from 1. No treatment added (Black) and 2. Melanoma cell addition (Blue). Created with BioRender.com by Dayna Spurling; Reprinted/adapted with permission from Ref. . 2019, Anchan, A.

Article Snippet: To assess the strength of the cerebral endothelial barrier, hCMVECs were grown on gold-plated 96-well ECIS plates (Applied Biophysics, Troy, NY, USA).

Techniques:

Human cerebral microvascular endothelial cell CD144 staining after NZM7-S treatment at varied concentrations. hCMVECs were seeded at 20,000 cells per well. NZM7-S were live stained with CytoTrack Red (CTR; red in image) and applied to the apical face of a confluent endothelial monolayer. Cells were co-cultured for 4 h, washed and then fixed. Junctions were stained with anti-CD144 (green). ( A ) An image from the media control well. ( B ) A paired ECIS experiment, where the boxed region shows the point of fixing for the ICC images (4 h). ( C , D,E ) The images of the melanoma-treated wells at different E:T ratios, noted on the left side of the panel. Images were acquired on the Operetta CLS imaging system. Full view images scale bar = 100 µm. Images are representative of 2 independent experiments.

Journal: International Journal of Molecular Sciences

Article Title: Melanoma Cells Produce Large Vesicular-Bodies That Cause Rapid Disruption of Brain Endothelial Barrier-Integrity and Disassembly of Junctional Proteins

doi: 10.3390/ijms24076082

Figure Lengend Snippet: Human cerebral microvascular endothelial cell CD144 staining after NZM7-S treatment at varied concentrations. hCMVECs were seeded at 20,000 cells per well. NZM7-S were live stained with CytoTrack Red (CTR; red in image) and applied to the apical face of a confluent endothelial monolayer. Cells were co-cultured for 4 h, washed and then fixed. Junctions were stained with anti-CD144 (green). ( A ) An image from the media control well. ( B ) A paired ECIS experiment, where the boxed region shows the point of fixing for the ICC images (4 h). ( C , D,E ) The images of the melanoma-treated wells at different E:T ratios, noted on the left side of the panel. Images were acquired on the Operetta CLS imaging system. Full view images scale bar = 100 µm. Images are representative of 2 independent experiments.

Article Snippet: To assess the strength of the cerebral endothelial barrier, hCMVECs were grown on gold-plated 96-well ECIS plates (Applied Biophysics, Troy, NY, USA).

Techniques: Staining, Cell Culture, Imaging